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1 article(s) from Wolfram, Stefanie

A small azide-modified thiazole-based reporter molecule for fluorescence and mass spectrometric detection

Stefanie Wolfram,
Hendryk Würfel,
Stefanie H. Habenicht,
Christine Lembke,
Phillipp Richter,
Eckhard Birckner,
Rainer Beckert and
Georg Pohnert

Beilstein J. Org. Chem. 2014, 10, 2470–2479, doi:10.3762/bjoc.10.258

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Graphical Abstract

Figure 1: Structure of the reporter molecule BPT (1).

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Scheme 1: Synthesis of the azide-bearing 4-hydroxythiazole derivative 1.

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Figure 2: Structure of the tested azide-modified standard fluorophores DNS (8) and NBD (9) and the bromine mo...

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Figure 3: UV–vis spectra of 20 µM solutions of the azide modified fluorophores BPT (1), DNS (8), NBD (9) and ...

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Figure 4: Normalized absorbance and fluorescence of BPT (1) in 20% THF/80% water (v/v), excitation at 374 nm.

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Figure 5: Peak area of 100 pmol BPT (1), DNS (8), NBD (9) and BNS (6) measured with (A) C18-UPLC coupled to a...

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Figure 6: Procedure of the model reaction between L-lysine and DDY (10) to form an imine (only one of two pos...

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Figure 7: Mass spectra of labeled L-lysine/DDY (10)/fluorophore conjugates 11 (containing BPT), 12 (containin...

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Figure 8: Fluorescent labeling of catalase treated with DDY (10) followed by CuAAC with all four reporter mol...

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Published 23 Oct 2014

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